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Berlin Center for Integrative Structural Biology (BIS)

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Structural Biology is a key discipline of modern Molecular and Medical Biology and a cornerstone of all major research consortia in the Berlin area. The Life Science Campus Berlin offers state-of-the-art, large research infrastructure for Structural Biology via the EM Ultra-Structure Network (Charité, FUB, MPI-MG), Joint Berlin MX-Laboratory (HZB, FUB, HUB, MDC, FMP, Charité), MS-based Structural Interactomics facilities (TUB, FMP, FUB), and the NMR centers (FMP, FUB). This infrastructure enables cutting-edge Structural Biology research through complementary approaches. To further reinforce long-term, close collaboration, we aim at integrating the existing collaborative networks into a newly formed Berlin Center for Integrative Structural Biology (BIS) to serve as a platform for the operation, further development and usage of this unique collection of equipment for the Berlin Structural Biology community.

Basic Information

8 Member Institutions:

  • Freie Universität Berlin (FUB)
  • Humboldt Universität zu Berlin (HUB)
  • Technische Universität Berlin (TUB)
  • Charité – Universitätsmedizin Berlin (Charité)
  • Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin
  • Helmholtz-Zentrum Berlin für Materialien und Energie (HZB)
  • Max Delbrück Center for Molecular Medicine (MDC), Berlin
  • Max Planck Institute for Molecular Genetics (MPI-MG), Berlin

Key Units:

  • EM Ultra-Structure Network (Charité, FUB, MPI-MG)
  • Joint Berlin MX-Laboratory (HZB, FUB, HUB, MDC, FMP, Charité)
  • MS-based Structural Interactomics facilities (TUB, FMP, FUB)
  • NMR centers (FMP, FUB)

Transmission cryo-electron microscopy

(FUB, Charité, MDC, FMP, MPI-MG)

Freie Universität Berlin (FUB)

Cryo-TEMs

  • Thermo Scientific 300 kV Krios G3i (single-particle)
  • FEI 200 kV Talos Arctica
  • FEI 200 kV Tecnai F20
  • FEI 120 KV Talos L120

Charité - Universitätsmedizin Berlin (Charité)

Cryo-TEMs

  • Thermo Scientific 300 kV Krios G3i (tomography)
  • FEI 200 kV Tecnai 20 TWIN

Max Planck Institute for Molecular Genetics (MPI-MG)

Cryo-TEMs

  • FEI 300 kV Tecnai G2 Polara
  • FEI 120 kV Tecnai Spirit

Joint Berlin MX-Laboratory

(HZB, FUB, HUB, MDC, FMP, Charité)

Operation, usage and further development of BESSY II MX beamlines

  • Founded 2009
  • Phase III 2018-2020
  • Annual meeting of the Berlin Structural Biology community (Joint Berlin MX-Day)

Beamlines at the BESSY II

Beamline 14.1

  • Energy range 5.5 – 15.5 keV
  • 5.0 x 1011 photons/s
  • High performance multi-axis goniometer MD2
  • Automated sample mounting
  • PILATUS 6M-detector
  • High performance de novo structure determination by MAD/SAD

Beamline 14.2

  • Energy range 5.5 – 15.5 keV
  • 4.6 x 1011 photons/s
  • Nanodiffractometer
  • Automated sample mounting
  • PILATUS3S 2M-detector
  • De novo structure determination by MAD/SAD, long-wavelength S-SAD

Beamline 14.3

  • Energy 13.8 keV
  • 6.8 x 1010 photons/s
  • High performance multi-axis goniometer MD2-S
  • Rayonix MX225 CCD-detector
  • In situ crystal Screening
  • Crystal dehydration experiments
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